Journal: BMC Plant Biology

Article Title: Transcriptomic analysis of wound xylem formation in Pinus canariensis

doi: 10.1186/s12870-017-1183-3

Figure Lengend Snippet: qRT-PCR validation of microarray transcription profiles. X-axis: sampled times; Y-axis: normalized gene expression values of selected DEGs for qRT-PCR (bars) validation of microarray expression profiling (continuous lines)

Article Snippet: A set of 15266 contigs involved in meristematic activity of Pinus canariensis , selected from a previous work [ ], was used for the design of a two-color 60K microarray (Agilent, USA).

Techniques: Quantitative RT-PCR, Microarray, Expressing

Journal: Plant Direct

Article Title: Overexpression of exogenous biuret hydrolase in rice plants confers tolerance to biuret toxicity

doi: 10.1002/pld3.290

Figure Lengend Snippet: Microarray‐based transcriptome analysis in rice suspension cells under biuret toxicity. (a) Biuret toxicity in rice suspension cells. Cells were subcultured into a culture medium supplemented with 0, 0.1, 0.3, and 1.0 mmol/L biuret. Fresh cell weight per culture flask was measured seven days after subcloning. Values are expressed means ± SD ( n = 3). Different alphabets indicate significant differences among groups ( p < 0.05, Tukey's test). (b) Changes in the fresh cell weight over time. Rice cells were subcultured into the culture medium supplemented with 0 and 0.3 mmol/L biuret on day 0. The cells on day three and day 5 were used for subsequent microarray analyses. White circles indicate the control cells, and black circles indicate biuret‐treated cells. Values are expressed means ± SD ( n = 2). An asterisk indicates a significant difference between the treatments ( p < 0.05, t test). (c) Relationship between the normalized average gene expression levels and fold changes of gene expression of the 3‐day‐old biuret‐treated cells to the control cells. Each symbol denotes each microarray spot. Values are geometrical means of two arrays ( n = 2). (d) Relationship between the normalized average gene expression levels and fold changes of gene expression of the 5‐day‐old biuret‐treated cells to the control cells. Each symbol denotes each microarray spot. Eleven points with high non‐significant fold‐change are omitted from the figure. Values are geometrical means of two arrays ( n = 2). (e) Numbers of differentially expressed genes

Article Snippet: Two‐color microarray analysis with two biological replicates was performed using Agilent Rice Oligo DNA Microarray 4x44K slide (Agilent, Santa Clara, CA, USA) according to the manufacturer's instructions to estimate the ratio of transcript abundance between the treatments at each culture period.

Techniques: Microarray, Subcloning, Expressing